Western blot was performed using Anti-TGFBR2 Polyclonal Antibody (Product # PA5-47719) and a 65 kDa band corresponding to TGFBR2 was observed across tissue extracts tested except Mouse Tongue and Rat tongue which is reported to be low. Uncharacterized bands around 48 kDa and 20 kDa were also observed in the samples tested. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Lung (Lane 3), Rat Lung (Lane 4), Mouse Tongue (Lane 5) and Rat Tongue (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).